Transcriptome profiling uncovers the involvement of CmXyn1, a glycosyl hydrolase 11, in Cochliobolus miyabeanus pathogenicity

Abstract Necrotrophic pathogen Cochliobolus miyabeanus ( C. ) causes rice brown leaf spot disease and drastically affects the yield quality of grains. However, molecular mechanism rice- remains poorly understood due to limited research conducted on this pathosystem. To elucidate , a transcriptome analysis was from in vitro planta grown miyabeanus. This led identification total 24,060 genes which 426 57 expressed were predicted encode for secretory proteins. As these specifically be nature, consider as putative effectors, highlighting their possible roles fungal pathogenicity. Notably, among CmXyn1 encodes glycosyl hydrolase 11 displayed highest expression level under conditions thus selected further functional characterization. Interestingly, extracellular transiently induced cell death Nicotiana benthamiana leaves, while intracellular comparatively lesser effective. In addition, leaves during infection comparing it data obtained hemibiotrophic Magnaporthe oryzae discovery 18 receptors/receptor-like kinases that commonly response both pathogens, indicating key defense response. Taken together, our findings provide new insights into interaction well unique common responses against hemibiotroph necrotroph model systems.